RIPA Lysis Buffer (Weak)

RIPA Lysis Buffer (Weak) is a cell and tissue lysis buffer, commonly used in Western blot and Immunoprecipitation applications. This buffer is most effective for Immunoprecipitation (IP) protocols, to keep proteins folded/active.

Abbexa's RIPA (Radio Immunoprecipitation Assay) Lysis Buffers are available in different strengths to balance protein extraction with structure and function:

1. abx090624 - Strong (high detergent concentration)
2. abx090624 - Medium (lower detergent concentration)
3. abx090626 - Weak (very low detergent concentration)

Protein concentration following lysis can be determined by our BCA Protein Assay Kits (abx090640, abx090641, abx090642 and abx090643). It is not recommended to use a Bradford Protein Assay Kit or other Kinase assay kits when using a strong RIPA buffer.

Target	RIPA Lysis Buffer
Research Area	Cell Lysis Buffers
Tested Applications	WB, IP
Form	Liquid
Storage	Store at 4°C.
Shelf Life	12 months.
Buffer	Cotnains 50mM Tris, pH 7.4, 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, and inhibitors (sodium orthovanadate, sodium fluoride, EDTA and leupeptin).
Availability	Shipped within 5-10 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on RIPA Lysis Buffer

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