RNA Purification Kit

RNA Purification Kit uses silica member-based spin column for specific RNA binding. It is suitable for simple and fast purification of DNase I-treated total RNA, in vitro transcription product, RNA-labelled product, synthetic RNA. This kit permits effective removal of proteins, organic chemicals, inorganic saline ions. Purified RNA can be used in RT-PCR, qRT-PCR, chip analysis, Northern Blot and RNAi.

Kit contents (25 rxns):

• Binding Buffer: 10 ml
• Wash Buffer: 8 ml
• RNase-free Water: 1.5 ml
• RNase-free Tube (1.5 ml): 25
• RNA Spin Columns with Collection Tubes: 25

Target	RNA Purification Kit
Storage	Store at room temperature.
Shelf Life	12 months.
Availability	Shipped within 10-20 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use	To prepare the Working Wash Buffer, add 32 ml of 96-100% ethanol to 8 ml Wash Buffer. Prepare only prior to immediate use. To prepare the Working Binding Buffer, add 10 µl beta-mercaptoethanol for each 1 ml Binding buffer. Prepare only prior to immediate use. Transfer ≤ 100 µg RNA sample into a microcentrifuge tube and add 100 µl RNase-free water. Add 350 µl of Working Binding Buffer (with beta-mercaptoethanol). Mix thoroughly by inverting or vortexing. Add 900 µl of 96-100% ethanol. Precipitates may form at this stage. Mix thoroughly by inverting or vortexing. Transfer half the volume of the solution (and any precipitates) to a spin column. Centrifuge at 12,000 × g for 30 seconds at room temperature. Discard the flow-through. Repeat the step above with the remaining half volume of solution. Add 500 µl of Working Wash Buffer (with ethanol). Centrifuge at 12,000 × g for 30 seconds. Discard the flow-through. Repeat the step above once more. Centrifuge at 12,000 × g for 2 minutes at room temperature. Air-dry the column matrix for several minutes. Place the spin column into a clean 1.5 ml RNase-free tube. Add 15-50 µl of RNase-free Water into the spin column matrix and leave the column to stand at room temperature for 1 minute. Centrifuge at 12,000 × g for 1 minute to elute the DNA. Store the purified RNA at -80°C.

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