SARS-CoV-2 Spike Glycorotein (Omicron B.1.1.529 Variant)

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Spike Glycoprotein, Omicron Variant is a full length soluble with foldon trimerization motif, mutated Furin recognition site and 6 stabilising mutations (F817P, A892P, A899P, A942P, K986P and V987P), based on/modified from Amanat et al, 2020 and Hsiehet al, 2020.

The SARS-CoV-2 Spike Protein (S protein) is a viral protein that allows the entry of SARS-CoV-2 into human cells. The protein forms trimers on the viral capsid and binds to human Angiotensin Converting Enzyme 2 (ACE2) located on the cell surface. The protein has a cleavage site between the S1 and S2 subunits that is targeted by the human enzyme Furin, and it may also cause the development of a syncytium (cell fusion). Antibodies to S protein can prevent viral entry as well as target the virus for further immune action.

The B.1.1.529 (Omicron) variant was first identified in South Africa. It has many concerning spike protein substitutions, some of which are known from other variants to be associated with reduced susceptibility to available monoclonal antibody therapeutics or reduced neutralization by convalescent and vaccinee sera.

Target	SARS-CoV-2 Spike Glycoprotein
Research Area	Infection Immunity
Origin	Human
Expression	Recombinant
Tested Applications	SDS-PAGE, WB
Host	HEK293 cells
Conjugation	Unconjugated
Form	Liquid
Purity	> 90% (SDS-PAGE)
Purification	Purified by affinity chromatography.
Storage	Aliquot and store between -20°C and -70°C. Avoid repeated freeze/thaw cycles.
Shelf Life	12 months.
Stability	Stable for at least 1 month when stored between 2°C and 8°C, or for at least 12 months when stored between -20°C and -70°C.
UniProt Primary AC	P0DTC2 (UniProt, ExPASy)
UniProt Entry Name	SPIKE_SARS2
Gene Symbol	S
GeneID	43740568
KEGG	vg:43740568
Molecular Weight	Calculated MW: 137 kDa Observed MW (SDS-PAGE): 190 kDa
Sequence Fragment	16-1211 AA.
Sequence	VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAISGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIDDTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSHRGAGSVASQSIIAYTMSLGAENSVAYSNNSIAIPINFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGPALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILARLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTHNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKGSGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGSGHHHHHHHH
Buffer	PBS, containing 20% glycerol.
Endotoxin Level	< 0.1 ng/µg (1 IEU/µg), as measured by LAL method.
Availability	Shipped within 5-7 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on SARS-CoV-2 Spike Glycoprotein

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