SARS-CoV-2 Spike Glycoprotein (D614G Mutation)
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Catalogue No: abx169319
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(Size: 100 µg)
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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Spike Glycoprotein, D614G Variant is a full-length recombinant protein expressed in HEK293 cells. It contains the foldon trimerization motif, mutated Furin recognition site and 2 stabilising mutations (K986P and V987P), based on/modified from Amanat et al, 2020. The SARS-CoV-2 Spike Protein (S protein) is a viral protein that allows the entry of SARS-CoV-2 into human cells. The protein forms trimers on the viral capsid and binds to human Angiotensin Converting Enzyme 2 (ACE2) located on the cell surface. The protein has a cleavage site between the S1 and S2 subunits that is targeted by the human enzyme Furin, and it may also cause the development of a syncytium (cell fusion). Antibodies to S protein can prevent viral entry as well as target the virus for further immune action.
Research Articles on SARS-CoV-2 Spike Glycoprotein
| Target | SARS-CoV-2 Spike Glycoprotein |
| Research Area | Infection Immunity |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE, WB |
| Host | HEK293 cells |
| Conjugation | Unconjugated |
| Form | Liquid |
| Purity | > 90% (SDS-PAGE) |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store between -20°C and -80°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| Stability | Stable for at least 1 month when stored between 2°C and 8°C, or for at least 12 months when stored between -20°C and -80°C. |
| UniProt Primary AC | P0DTC2 (UniProt, ExPASy) |
| UniProt Entry Name | SPIKE_SARS2 |
| Gene Symbol | S |
| GeneID | 43740568 |
| KEGG | vg:43740568 |
| Molecular Weight | Calculated MW: 137 kDa Observed MW (SDS-PAGE): 190 kDa |
| Sequence Fragment | 16-1213 AA. |
| Sequence | VNLTTRTQLP PAYTNSFTRG VYYPDKVFRS SVLHSTQDLF LPFFSNVTWF HAIHVSGTNG TKRFDNPVLP FNDGVYFAST EKSNIIRGWI FGTTLDSKTQ SLLIVNNATN VVIKVCEFQF CNDPFLGVYY HKNNKSWMES EFRVYSSANN CTFEYVSQPF LMDLEGKQGN FKNLREFVFK NIDGYFKIYS KHTPINLVRD LPQGFSALEP LVDLPIGINI TRFQTLLALH RSYLTPGDSS SGWTAGAAAY YVGYLQPRTF LLKYNENGTI TDAVDCALDP LSETKCTLKS FTVEKGIYQT SNFRVQPTES IVRFPNITNL CPFGEVFNAT RFASVYAWNR KRISNCVADY SVLYNSASFS TFKCYGVSPT KLNDLCFTNV YADSFVIRGD EVRQIAPGQT GKIADYNYKL PDDFTGCVIA WNSNNLDSKV GGNYNYLYRL FRKSNLKPFE RDISTEIYQA GSTPCNGVEG FNCYFPLQSY GFQPTNGVGY QPYRVVVLSF ELLHAPATVC GPKKSTNLVK NKCVNFNFNG LTGTGVLTES NKKFLPFQQF GRDIADTTDA VRDPQTLEIL DITPCSFGGV SVITPGTNTS NQVAVLYQGV NCTEVPVAIH ADQLTPTWRV YSTGSNVFQT RAGCLIGAEH VNNSYECDIP IGAGICASYQ TQTNSPGGAS VASQSIIAYT MSLGAENSVA YSNNSIAIPT NFTISVTTEI LPVSMTKTSV DCTMYICGDS TECSNLLLQY GSFCTQLNRA LTGIAVEQDK NTQEVFAQVK QIYKTPPIKD FGGFNFSQIL PDPSKPSKRS FIEDLLFNKV TLADAGFIKQ YGDCLGDIAA RDLICAQKFN GLTVLPPLLT DEMIAQYTSA LLAGTITSGW TFGAGAALQI PFAMQMAYRF NGIGVTQNVL YENQKLIANQ FNSAIGKIQD SLSSTASALG KLQDVVNQNA QALNTLVKQL SSNFGAISSV LNDILSRLDP PEAEVQIDRL ITGRLQSLQT YVTQQLIRAA EIRASANLAA TKMSECVLGQ SKRVDFCGKG YHLMSFPQSA PHGVVFLHVT YVPAQEKNFT TAPAICHDGK AHFPREGVFV SNGTHWFVTQ RNFYEPQIIT TDNTFVSGNC DVVIGIVNNT VYDPLQPELD SFKEELDKYF KNHTSPDVDL GDISGINASV VNIQKEIDRL NEVAKNLNES LIDLQELGKY EQYIKWPSGG SGYIPEAPRD GQAYVRKDGE WVLLSTFLGS HHHHHHHH |
| Tag | C-terminal 8 His tag |
| Buffer | PBS, containing 20% glycerol. |
| Endotoxin Level | < 0.1 ng/µg (1 IEU/µg), as measured by LAL method. |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |