SARS-CoV-2 Spike Protein

SARS-CoV-2/COVID-19 Spike Protein is a recombinant protein expressed in E. coli.

The SARS-CoV-2 Spike Protein (S protein) is a viral protein that allows the entry of SARS-CoV-2 into human cells. The protein forms trimers on the viral capsid and binds to human Angiotensin Converting Enzyme 2 (ACE2) located on the cell surface. The protein has a cleavage site between the S1 and S2 subunits that is targeted by the human enzyme Furin, and it may also cause the development of a syncytium (cell fusion). Antibodies to S protein can prevent viral entry as well as target the virus for further immune action.

Target	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spike Glycoprotein
Origin	Virus
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 90%
Reconstitution	Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 29.5 kDa Observed MW (SDS-PAGE): 29 kDa
Sequence Fragment	Arg319-Phe541
Sequence	RV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YLYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN F
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity	Not tested
Concentration	Prior to lyophilization: 100 µg/ml
Availability	Shipped within 5-15 working days.
Note	This product is for research use only.

Research Articles on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spike Glycoprotein

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