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SARS-CoV-2/COVID-19 Spike Protein is a recombinant protein expressed in E. coli.
The SARS-CoV-2 Spike Protein (S protein) is a viral protein that allows the entry of SARS-CoV-2 into human cells. The protein forms trimers on the viral capsid and binds to human Angiotensin Converting Enzyme 2 (ACE2) located on the cell surface. The protein has a cleavage site between the S1 and S2 subunits that is targeted by the human enzyme Furin, and it may also cause the development of a syncytium (cell fusion). Antibodies to S protein can prevent viral entry as well as target the virus for further immune action.
| Target |
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Spike Glycoprotein |
| Origin |
Virus |
| Expression |
Recombinant |
| Tested Applications |
WB, SDS-PAGE |
| Host |
E. coli |
| Conjugation |
Unconjugated |
| Form |
Lyophilized |
| Purity |
> 90% |
| Reconstitution |
Reconstitute to the original concentration in ddH2O. If further dilutions are required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0, to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
| Storage |
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
Calculated MW: 29.5 kDa
Observed MW (SDS-PAGE): 29 kDa |
| Sequence Fragment |
Arg319-Phe541 |
| Sequence |
RV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YLYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN F |
| Tag |
N-terminal His Tag |
| Buffer |
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Activity |
Not tested |
| Concentration |
Prior to lyophilization: 200 µg/ml |
| Availability |
Shipped within 5-15 working days. |
| Note |
This product is for research use only. |
Research Articles on SARS-CoV-2/COVID-19 Spike Protein
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