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SDS-PAGE Gel Preparation Kit
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Catalogue No: abx090646
Price:
US$159.50
(Size: 1 Kit)
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Datasheet
Abbexa's SDS-PAGE Gel Preparation Kit contains reagents for preparation of 30-50 pieces of normal-sized PAGE gel. This kit can be used to prepare SDS-PAGE gel and native PAGE gel. Kit components:
Preparing SDS-PAGE separation gel (for native PAGE gel, do not add 10% SDS):
Preparing SDS-PAGE spacer gel:
Research Articles on SDS-PAGE Gel Preparation Kit
- 30% Acr-Bis (29:1): 100 ml
- 1.5 M Tris-HCl (pH 8.8): 100 ml
- 10% SDS: 5 ml
- Ammonium persulfate: 0.5 g
- TEMED: 0.5 ml
- 1.0 M Tris-HCl (pH 6.8): 15 ml
| SDS-PAGE Separation Gel Concentration | Molecular Weight of Target Protein |
| 6% Gel | 50-150 kDa |
| 8% Gel | 30-90 kDa |
| 10% Gel | 20-80 kDa |
| 12% Gel | 12-60 kDa |
| 15% Gel | 10-40 kDa |
| Component | Volume (ml) | |||||
| 6% Gel | 5 | 10 | 15 | 20 | 30 | 50 |
| Double-distilled water | 2.6 | 5.3 | 7.9 | 10.5 | 15.8 | 26.3 |
| 30% Acr-Bis (29:1) | 1.0 | 2.0 | 3.0 | 4.0 | 6.0 | 10.0 |
| 1.5 M Tris (pH 8.8) | 1.3 | 2.5 | 3.8 | 5.1 | 7.6 | 12.7 |
| 10% SDS | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| 10% Ammonium persulfate | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| TEMED | 0.004 | 0.008 | 0.012 | 0.016 | 0.024 | 0.040 |
| Component | Volume (ml) | |||||
| 8% Gel | 5 | 10 | 15 | 20 | 30 | 50 |
| Double-distilled water | 2.3 | 4.6 | 6.9 | 9.2 | 13.8 | 23.0 |
| 30% Acr-Bis (29:1) | 1.3 | 2.7 | 4.0 | 5.3 | 8.0 | 13.3 |
| 1.5 M Tris (pH 8.8) | 1.3 | 2.5 | 3.8 | 5.1 | 7.6 | 12.7 |
| 10% SDS | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| 10% Ammonium persulfate | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| TEMED | 0.003 | 0.006 | 0.009 | 0.012 | 0.018 | 0.030 |
| Component | Volume (ml) | |||||
| 10% Gel | 5 | 10 | 15 | 20 | 30 | 50 |
| Double-distilled water | 1.9 | 4.0 | 5.9 | 7.8 | 11.8 | 19.6 |
| 30% Acr-Bis (29:1) | 1.7 | 3.3 | 5.0 | 6.7 | 10.0 | 16.7 |
| 1.5 M Tris (pH 8.8) | 1.3 | 2.5 | 3.8 | 5.1 | 7.6 | 12.7 |
| 10% SDS | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| 10% Ammonium persulfate | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| TEMED | 0.002 | 0.004 | 0.006 | 0.008 | 0.012 | 0.020 |
| Component | Volume (ml) | |||||
| 12% Gel | 5 | 10 | 15 | 20 | 30 | 50 |
| Double-distilled water | 1.6 | 3.3 | 4.9 | 6.5 | 9.8 | 16.3 |
| 30% Acr-Bis (29:1) | 2.0 | 4.0 | 6.0 | 8.0 | 12.0 | 20.0 |
| 1.5 M Tris (pH 8.8) | 1.3 | 2.5 | 3.8 | 5.1 | 7.6 | 12.7 |
| 10% SDS | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| 10% Ammonium persulfate | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| TEMED | 0.002 | 0.004 | 0.006 | 0.008 | 0.012 | 0.020 |
| Component | Volume (ml) | |||||
| 15% Gel | 5 | 10 | 15 | 20 | 30 | 50 |
| Double-distilled water | 1.1 | 2.3 | 3.4 | 4.5 | 6.8 | 11.3 |
| 30% Acr-Bis (29:1) | 2.5 | 5.0 | 7.5 | 10.0 | 15.0 | 25.0 |
| 1.5 M Tris (pH 8.8) | 1.3 | 2.5 | 3.8 | 5.1 | 7.6 | 12.7 |
| 10% SDS | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| 10% Ammonium persulfate | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.50 |
| TEMED | 0.002 | 0.004 | 0.006 | 0.008 | 0.012 | 0.020 |
| Component | Volume (ml) | |||||
| 5% Gel | 2 | 3 | 4 | 6 | 8 | 10 |
| Double-distilled water | 1.4 | 2.1 | 2.7 | 4.1 | 5.5 | 6.8 |
| 30% Acr-Bis (29:1) | 0.33 | 0.50 | 0.67 | 1.00 | 1.30 | 1.70 |
| 1 M Tris (pH 6.8) | 0.25 | 0.38 | 0.50 | 0.75 | 1.00 | 1.25 |
| 10% SDS | 0.02 | 0.03 | 0.04 | 0.06 | 0.08 | 0.10 |
| 10% Ammonium persulfate | 0.02 | 0.03 | 0.04 | 0.06 | 0.08 | 0.10 |
| TEMED | 0.002 | 0.003 | 0.004 | 0.006 | 0.008 | 0.010 |
| Tested Applications | WB |
| Storage | Store all reagents at 4 °C. 30% Acr-Bis and TEMED should be stored in the dark. Before use, prepare a 10% Ammonium persulfate solution using double-distilled water, which can then be aliquoted and stored at -20 °C for up to 6 months. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |
