SDS-PAGE Loading Buffer (2X)

Name: SDS-PAGE Loading Buffer (2X)
Brand: Abbexa
SKU: abx090647
Price: 90.0000 GBP
Availability: OnlineOnly

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Catalogue No: abx090647

Price: US$130.50

(Size: 5 ml)

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* Size: 5 ml

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SDS-PAGE Sample Loading Buffer is a 2X concentrated solution based on Bromophenol blue dye for use in SDS-PAGE protein sample loading.

Tested Applications	WB
Storage	Store at -20 °C for up to one year.
Buffer	Contains Tris-HCl buffer, SDS, DTT, Bromophenol Blue and glycerol.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.
Directions for use	Allow to stand in a water bath at a temperature between room temperature and 37 °C until all precipitates/sediments have dissolved. Then, allow to stand at room temperature. Add sample to the 2X SDS-PAGE Sample Loading Buffer in a ratio of 1:1 (e.g. add 1 µl of sample to 1 µl of 2X SDS-PAGE Sample Loading Buffer). Fully denature the sample at 100 °C or in a boiling water bath for 3-5 min. Cool the sample to room temperature, then load and carry out electrophoresis. Stop electrophoresis when the bromophenol blue dye reaches the bottom of the gel. Notes: If SDS sediments appear in the SDS-PAGE Sample Loading Buffer at room temperature, they can be dissolved using a warm water bath. This product contains DTT, which can have a distinct odour. Personal protective equipment (PPE), such as lab coats, lab glasses and disposable gloves, are recommended when using this product.

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