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Serratia marcescens Endonuclease Enzyme
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Catalogue No: abx073081
Price:
US$304.50
(Size: 5 kU)
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Serratia Marcescens Endonuclease is a recombinant enzyme expressed in E. coli.
Research Articles on Endonuclease
| Target | Endonuclease |
| Origin | Bacteria |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | E. coli |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
| Purity | > 99% (SDS-PAGE) |
| Purification | Purified by proprietary chromatographic techniques. |
| Storage | Store at 4 °C if the entire vial will be used within 4 weeks. Store at -20 °C for long-term storage. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles. |
| Molecular Weight | 30 kDa |
| Buffer | 50 mM Tris-HCl, pH 8.0, containing 20 mM NaCl, 2 mM MgCl2 and 50% glycerol. |
| Biological Activity | 1 Unit is defined as the amount of enzyme that causes a ΔA260 of 1 in 30 min, which corresponds to complete digestion of 37 µg DNA. Standard reaction conditions are 1 mg/ml sonicated DNA substrate in 50 mM Tris-HCl, pH 8.0, 0.1 mg/ml BSA, 1 mM MgCl2, incubated at 37 °C. |
| Specificity | Unspecific (DNA, RNA) attacks all nucleic acids (single strand, double strand, circular, supercoiled) with no apparent sequence preference. Final reaction product: 5'-mono-phosphate terminated oligonucleotides (3-5 bases). Protease Activity: Not detectable. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
