Serum Albumin Assay Kit (Bromocresol Green Method)
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Price:
US$188.50
(Size: 96 tests)
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Serum Albumin Assay Kit (Bromocresol Green Method) is an assay kit by Bromocresol Green Method.
Kit Components: - Reagent A (Bromocresol Green Solution): 5 ml (5X concentrated)
- Reagent B (Albumin Standard): 0.3 ml (34.8 g/L)
| Target |
Serum Albumin Assay Kit (Bromocresol Green Method) |
| Recommended dilutions |
Serum samples: 1/10. Optimal dilutions/concentrations should be determined by the end user. |
| Storage |
Store at 4 °C in the dark. |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |
| Directions for use |
Assay Procedure: - Prepare the Bromocresol Green Working Solution by diluting the 5X concentrated Bromocresol Green Solution with double-distilled water at a ratio of 1:4 (i.e. add 5 ml of 5X concentrated Bromocresol Green Solution to 20 ml of double-distilled water).
- Prepare the Albumin Standard Working Solution (3.48 g/L) by diluting the Albumin Standard Solution (34.8 g/L) with double-distilled water at a ratio of 1:9 (i.e. add 0.3 ml of Albumin Standard Solution to 2.7 ml of double-distilled water).
- Set the sample, standard and blank tubes. We recommend setting each standard and sample in duplicate.
- Add 10 µl of double-distilled water to each blank tube.
- Add 10 µl of Albumin Standard Working Solution to each standard tube.
- Add 10 µl of sample to each sample tube.
- Add 250 µl of Bromocresol Green Working Solution to each tube. Mix thoroughly.
- Allow the tubes to stand at room temperature for 10 minutes, then read and record the absorbance at 628 nm or 630 nm.
- Calculate the albumin concentration as follows:
Albumin Concentration (g/L) = [OD of Sample - OD of Blank] / [OD of Standard - OD of Blank] × Concentration of Albumin Standard Working Solution (3.48 g/L)
Standard Curve: - Set the standard tubes and dilute the 34.8 g/L standard to the following concentrations with double-distilled water: 13.92, 8.7, 6.96, 4.35, 3.48, 1.74, 0.87 and 0.435 g/L.
- To a tube, add 10 µl of prepared standard, 10 µl of distilled water, and 250 µl of Bromocresol Green Working Solution. Mix thoroughly
- Allow the tubes to stand at room temperature for 10 minutes, then read and record the absorbance at 628 nm or 630 nm.
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