Serum Albumin Assay Kit (Bromocresol Green Method)

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Catalogue No: abx090700
Price: US$188.50
(Size: 96 tests)

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Serum Albumin Assay Kit (Bromocresol Green Method)

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Datasheet SDS
Serum Albumin Assay Kit (Bromocresol Green Method) is an assay kit by Bromocresol Green Method.

Kit Components:
  • Reagent A (Bromocresol Green Solution): 5 ml (5X concentrated)
  • Reagent B (Albumin Standard): 0.3 ml (34.8 g/L)


Target Serum Albumin Assay Kit (Bromocresol Green Method)
Recommended dilutions Serum samples: 1/10. Optimal dilutions/concentrations should be determined by the end user.
Storage Store at 4 °C in the dark.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Assay Procedure:
  1. Prepare the Bromocresol Green Working Solution by diluting the 5X concentrated Bromocresol Green Solution with double-distilled water at a ratio of 1:4 (i.e. add 5 ml of 5X concentrated Bromocresol Green Solution to 20 ml of double-distilled water).
  2. Prepare the Albumin Standard Working Solution (3.48 g/L) by diluting the Albumin Standard Solution (34.8 g/L) with double-distilled water at a ratio of 1:9 (i.e. add 0.3 ml of Albumin Standard Solution to 2.7 ml of double-distilled water).
  3. Set the sample, standard and blank tubes. We recommend setting each standard and sample in duplicate.
  4. Add 10 µl of double-distilled water to each blank tube.
  5. Add 10 µl of Albumin Standard Working Solution to each standard tube.
  6. Add 10 µl of sample to each sample tube.
  7. Add 250 µl of Bromocresol Green Working Solution to each tube. Mix thoroughly.
  8. Allow the tubes to stand at room temperature for 10 minutes, then read and record the absorbance at 628 nm or 630 nm.
  9. Calculate the albumin concentration as follows:
    Albumin Concentration (g/L) = [OD of Sample - OD of Blank] / [OD of Standard - OD of Blank] × Concentration of Albumin Standard Working Solution (3.48 g/L)

Standard Curve:
  1. Set the standard tubes and dilute the 34.8 g/L standard to the following concentrations with double-distilled water: 13.92, 8.7, 6.96, 4.35, 3.48, 1.74, 0.87 and 0.435 g/L.
  2. To a tube, add 10 µl of prepared standard, 10 µl of distilled water, and 250 µl of Bromocresol Green Working Solution. Mix thoroughly
  3. Allow the tubes to stand at room temperature for 10 minutes, then read and record the absorbance at 628 nm or 630 nm.
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