Protein SET (SET) Antibody

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL) induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

Target	Protein SET (SET)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 47-75 amino acids from the N-terminal region of human SET.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q01105 (UniProt, ExPASy)
UniProt Secondary AC	A5A5H4, A6NGV1, B4DUE2, Q15541, Q5VXV1, Q5VXV2, Q6FHZ5
UniProt Entry Name	SET_HUMAN
KEGG	hsa:6418
String	9606.ENSP00000361777
Molecular Weight	Calculated MW: 33.5 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse, Rat and Drosophila SET.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Protein SET (SET)

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