SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100 to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.
Target |
Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5) |
Clonality |
Polyclonal |
Reactivity |
Human, Mouse |
Tested Applications |
ELISA, WB |
Host |
Rabbit |
Recommended dilutions |
WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation |
Unconjugated |
Immunogen |
KLH-conjugated synthetic peptide between 482-513 amino acids from the C-terminal region of human SIGLEC5. |
Isotype |
IgG |
Form |
Liquid |
Purification |
Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC |
O15389 (UniProt,
ExPASy)
|
UniProt Entry Name |
SIGL5_HUMAN |
Gene Symbol |
SIGLEC5 |
KEGG |
hsa:8778
|
String |
9606.ENSP00000470259
|
Molecular Weight |
Calculated MW: 60.7 kDa |
Buffer |
PBS containing 0.09% sodium azide. |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |
Research Articles on Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5)