Single-Step Dewaxing/Antigen Retrieval Buffer (pH 9)

The environment-friendly, Single-Step Dewaxing/Antigen Retrieval Buffer is a 20X concentrated rapid pretreatment reagent for paraffin slides. It replaces the traditional multistep process of dewaxing with only one reagent with comparatively shortened operation time. The steps to process the slides are designed in a simplified manner to reduce variability and enhance stability.

1. Dilute the Dewaxing/Antigen Retrieval Buffer (20X) with DI water to 1X Dewaxing/Antigen Retrieval working solution before use. For example: Take 30 ml Dewaxing/Antigen Retrieval Buffer (pH 9) (20X), dilute with DI water to 600 ml.

Volume of Dewaxing/Antigen Retrieval Buffer (20X)	Number of samples (PCS)
100 ml	≤140
200 ml	≤280
500 ml	≤700

2. Boil the 1X Dewaxing/Antigen Retrieval (pH 9) working solution in a glass repair box (using a water bath or microwave). Remove the repair box from the water bath or microwave and immerse the slides into the boiling solution (avoid using a metal slide rack).

3. Place the repair box containing the slides again in a water bath or microwave at medium-low power for 15-30 min to repair the antigen. Do not allow the tissue to get dry during the entire process.

4. Bring the repair box containing slides to RT to let it cool naturally and wash the slides with DI water (avoid breaking up the tissue).

Target	Single-Step Dewaxing/Antigen Retrieval Buffer
Tested Applications	IHC
Recommended dilutions	Dilute with DI water to 1X Dewaxing/Antigen Retrieval working solution before use.
Form	Liquid
Storage	Store at 2-8°C.
Validity	Up to 12 months.
Buffer	Not applicable.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.
Directions for use	Dilute with DI water to prepare 1X Dewaxing/Antigen Retrieval working solution before use.

Research Articles on Single-Step Dewaxing/Antigen Retrieval Buffer

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