Sirtuin 1 (SIRT1) Antibody

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Catalogue No: abx142225
Price: US$275.50
(Size: 50 µl)
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Immunofluorescent analysis of SIRT1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DL 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Sirtuin 1 (SIRT1) Antibody Sirtuin 1 (SIRT1) Antibody Sirtuin 1 (SIRT1) Antibody

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Datasheet SDS
SIRT1 Antibody is a Rabbit Polyclonal against SIRT1.

Target Sirtuin 1 (SIRT1)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications WB, IHC, IF/ICC, IP, ChIP
Host Rabbit
Recommended dilutions WB: 1/500 - 1/2000, IHC: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, IP: 1/50 - 1/200, ChIP: 1/20 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Immunogen KLH-conjugated synthetic peptide of human SIRT1.
Isotype IgG
Form Liquid
Purification Purified by immunogen affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q96EB6 (UniProt, ExPASy)
UniProt Secondary AC Q2XNF6, Q5JVQ0, Q9GZR9, Q9Y6F0
UniProt Entry Name SIR1_HUMAN
Gene Symbol SIRT1
GeneID 23411
KEGG hsa:23411
String 9606.ENSP00000212015
Buffer 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol and 0.01% sodium azide.
Specificity Recognizes endogenous levels of SIRT1 protein.
Concentration 1 mg/ml
Availability Shipped within 7-12 working days.
Note This product is for research use only.
Research Articles on Sirtuin 1 (SIRT1)


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