Mothers Against Decapentaplegic Homolog 2 Phospho-Thr220 (SMAD2 pT220) Antibody

SMAD2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF?receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8) ; the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.

Target	Mothers Against Decapentaplegic Homolog 2 Phospho-Thr220 (SMAD2 pT220)
Clonality	Polyclonal
Target Modification	Thr220
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, WB, IF/ICC, DB
Host	Rabbit
Recommended dilutions	WB: 1/500, IF/ICC: 1/10 - 1/50, DB: 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T220 of human SMAD2.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q15796 (UniProt, ExPASy)
UniProt Entry Name	SMAD2_HUMAN
NCBI Accession	NP_001003652.1 NP_005892.1
KEGG	hsa:4087
String	9606.ENSP00000262160
Molecular Weight	Calculated MW: 52.3 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse, Rat, Cow and Zebrafish SMAD2.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Mothers Against Decapentaplegic Homolog 2 Phospho-Thr220 (SMAD2 pT220)

Write a review

Your Name

Your Email Address

PMID

DOI

Your Review

Rating

Continue