Transcription Activator BRG1 (SMARCA4) Antibody

SMARCA4 Antibody is a Rabbit Polyclonal antibody against SMARCA4. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Target	Transcription Activator BRG1 (SMARCA4)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IHC, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, IP: 0.5-4 µg/200-400 µg whole cell extracts. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 680-770 of human BRG1/SMARCA4.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P51532 (UniProt, ExPASy)
UniProt Secondary AC	B1A8Z4, B1A8Z5, B1A8Z6, B1A8Z7, E9PBR8, O95052, Q9HBD3
UniProt Entry Name	SMCA4_HUMAN
Gene Symbol	SMARCA4
GeneID	6597
OMIM	603254
NCBI Accession	NP_003063.2
HGNC	11100
KEGG	hsa:6597
Ensembl	ENSG00000127616
String	9606.ENSP00000395654
Molecular Weight	Calculated MW: 185 kDa Observed MW: 220 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Transcription Activator BRG1 (SMARCA4)

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