Signal Transducer and Activator of Transcription 3 Phospho-Ser727 (STAT3 pS727) Antibody

STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.

Target	Signal Transducer and Activator of Transcription 3 Phospho-Ser727 (STAT3 pS727)
Clonality	Polyclonal
Target Modification	Ser727
Modification	Phosphorylation
Reactivity	Human, Mouse
Tested Applications	ELISA, WB, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/500, IF/ICC: 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P40763 (UniProt, ExPASy)
UniProt Secondary AC	A8K7B8, K7ENL3, O14916, Q9BW54
UniProt Entry Name	STAT3_HUMAN
KEGG	hsa:6774
String	9606.ENSP00000264657
Molecular Weight	Calculated MW: 88.1 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Signal Transducer and Activator of Transcription 3 Phospho-Ser727 (STAT3 pS727)

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