Signal Transducer and Activator of Transcription 5A Phospho-Tyr694 (STAT5A pY694) Antibody

STAT5a is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this protein is found to induce the expression of BCL2L1/BCL-X (L), which suggests the antiapoptotic function of this protein in cells.

Target	Signal Transducer and Activator of Transcription 5A Phospho-Tyr694 (STAT5A pY694)
Clonality	Polyclonal
Target Modification	Tyr694
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, WB, IHC, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100, IF/ICC: 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y694 of human STAT5a.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P42229 (UniProt, ExPASy)
UniProt Secondary AC	Q1KLZ6
UniProt Entry Name	STA5A_HUMAN
NCBI Accession	NP_003143.2
KEGG	hsa:6776
String	9606.ENSP00000341208
Molecular Weight	Calculated MW: 90.6 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Signal Transducer and Activator of Transcription 5A Phospho-Tyr694 (STAT5A pY694)

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