Transcription Elongation Factor SPT5 (SUPT5H) Antibody

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Target	Transcription Elongation Factor SPT5 (SUPT5H)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 395-424 amino acids from the Central region of human SUPT5H.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O00267 (UniProt, ExPASy)
UniProt Secondary AC	O43279, Q59G52, Q99639
UniProt Entry Name	SPT5H_HUMAN
Gene Symbol	SUPT5H
KEGG	hsa:6829
String	9606.ENSP00000470252
Molecular Weight	Calculated MW: 121 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse and Chicken SUPT5H.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Transcription Elongation Factor SPT5 (SUPT5H)

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