TATA Box Binding Protein (TBP) Antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

Target	TATA Box Binding Protein (TBP)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC, IF/ICC, FCM
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100, IF/ICC: 1/10 - 1/50, FCM: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 210-239 amino acids from the Central region of human TBP.
Isotype	IgG
Form	Liquid
Purification	Purified Rabbit Polyclonal Antibody.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P20226 (UniProt, ExPASy)
UniProt Secondary AC	B4E3B3, F5H869, Q16845, Q6IBM6, Q9UC02
UniProt Entry Name	TBP_HUMAN
Gene Symbol	TBP
NCBI Accession	NP_001165556.1 NP_003185.1
KEGG	hsa:6908
String	9606.ENSP00000375942
Molecular Weight	Calculated MW: 37.7 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse, Cow, Monkey, Zebrafish and Xenopus TBP.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on TATA Box Binding Protein (TBP)

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