Tight Junction Protein ZO-1 (TJP1) Antibody

TJP1 Antibody is a Rabbit Polyclonal antibody against TJP1 (ZO1). Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).

Target	Tight Junction Protein ZO-1 (TJP1)
Clonality	Polyclonal
Reactivity	Human, Rat
Tested Applications	WB, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 18-273 of human TJP1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q07157 (UniProt, ExPASy)
UniProt Secondary AC	B4E3K1, Q2NKP3, Q4ZGJ6
UniProt Entry Name	ZO1_HUMAN
Gene Symbol	TJP1
GeneID	7082
NCBI Accession	NP_003248.3
KEGG	hsa:7082
String	9606.ENSP00000281537
Molecular Weight	Calculated MW: 195 kDa Observed MW: 195 kDa
Buffer	PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration	1.26 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Tight Junction Protein ZO-1 (TJP1)

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