Rat Tissue Factor (TF) Protein (Active)

Rat Tissue Factor (TF) Protein (Active) is an active Rat protein expressed in E. coli.

Target	Tissue Factor (TF)
Origin	Rat
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P42533 (UniProt, ExPASy)
UniProt Entry Name	TF_RAT
String	10116.ENSRNOP00000015836
Molecular Weight	Calculated MW: 26.9 kDa Observed MW: 29 kDa
Sequence Fragment	Gly30-Glu252
Sequence	G TPPGKAFNLT WISTDFKTIL EWQPKPTNYT YTVQISDRSR NWKYKCTGTT DTECDLTDEI VKDVNWTYEA RVLSVPWRNS THGKETLFGT HGEEPPFTNA RKFLPYRDTK IGQPVIQKYE QGGTKLKVTV KDSFTLVRKN GTFLTLRQVF GNDLGYILTY RKDSSTGRKT NTTHTNEFLI DVEKGVSYCF FAQAVIFSRK TNHKSPESIT KCTEQWKSVL GE
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity	Active
Biological Activity	Tissue Factor (TF) is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is required for the initiation of thrombin formation from the zymogen prothrombin. It has been reported that Epidermal Growth Factor (EGF) can bind with the TF on the hemangioendotheliocytes, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EGF-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-TF polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of TF and EGF is shown in Figure 3.
Endotoxin Level	< 1.0 EU/µg (LAL method)
Concentration	Prior to lyophilization: 400 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

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Rat Tissue Factor (TF) Protein (Active)

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