TMB ELISA Substrate

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Catalogue No: abx098248
Price: US$319.00
(Size: 100 ml)
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TMB ELISA Substrate

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Datasheet
TMB ELISA Substrate is a ready-to-use chromogenic substrate for detection of horseradish peroxidase (HRP) activity. HRP can catalyze 3,3',5,5'-tetramethylbenzidine (TMB) to yield a blue color, the maximal absorbance is at 370 nm or 620-652 nm; however, upon addition of the stop solution, the solution turns to yellow and can be measured at 450 nm.

Target TMB ELISA Substrate
Tested Applications ELISA
Form Liquid
Storage Store 2-8 °C in the dark.
Buffer Contains 3,3',5,5'-Tetramethylbenzidine (TMB).
Availability Shipped within 10-20 working days.
Note This product is for research use only.
Directions for use Suggested Procedure
  1. For ELISA, after incubation with HRP-conjugated secondary antibody, wash the ELISA plate 3 times with PBST (PBS containing 0.05% Tween-20), 5 minutes each time.
  2. Add 100 µl of room-temperature TMB Solution to each well. Incubate for 3-30 minutes in the dark until the color of the solution has changed to the expected color. If a shorter reaction time is needed, this step can be carried out in a 37 °C incubator in the dark.
  3. (Optional) Add 100 µl of stop solution (0.5 M H2SO4 or 1 M HCl) to each well.
  4. Measure the absorbance at 370 nm or 620-650 nm (if no stop solution was added); or measure the absorbance at 450 nm (if stop solution was added).
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