TMB Substrate

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Catalogue No: abx299722
Price: US$522.00
(Size: 10 × 100 ml)
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TMB Substrate

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Datasheet
TMB Substrate is a ready-to-use reagent for use in ELISA. It reacts with horseradish peroxidase (HRP) to produce a blue colour. The reaction can be terminated by adding acidic stop solution (sulphuric or hydrochloric acid), which produces a stable yellow reaction product with absorbance maxima at 450 nm.

Target TMB Substrate
Tested Applications ELISA
Storage Store in the dark between 2-8 °C.
Buffer Contains 3,3',5,5'-Tetramethylbenzidine (TMB).
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use
  1. Add 100 µl of TMB to each microplate well.
  2. Incubate in the dark at 37 °C for 20-30 min. The reaction can be terminated when the desired color develops.
  3. Add 100 µl of stop solution (0.3 M H2SO4 or 0.25 M HCl) to each well.
  4. Read the absorbance of each well at 450 nm within 15 minutes.

Notes:
  • 0.1% NaF can be used as the stop solution instead of 0.3 M H2SO4 or 0.25 M HCl; the absorbance of each well should be read at 650 nm if using 0.1% NaF.
  • Avoid contamination with oxidizing reagents.
  • This product should be used in the dark. Do not use in metal containers.
  • High HRP concentrations may cause the TMB to turn a green or red color and may cause precipitation to occur. If this occurs, it is recommended to reduce the HRP concentration in the sample or reduce the incubation time.
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