TMB Substrate

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Catalogue No: abx299722
Price: US$522.00
(Size: 10 × 100 ml)

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TMB Substrate

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Datasheet SDS
TMB Substrate is a ready-to-use reagent for use in ELISA. It reacts with horseradish peroxidase (HRP) to produce a blue colour. The reaction can be terminated by adding acidic stop solution (sulphuric or hydrochloric acid), which produces a stable yellow reaction product.

Target TMB Substrate
Tested Applications ELISA
Storage Store at 2-8°. Avoid exposure to light.
Shelf Life 24 months.
Buffer Contains 3,3',5,5'-Tetramethylbenzidine (TMB).
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use
  1. Add 100 µl of TMB to each microplate well.
  2. Incubate in the dark at 37°C for 20-30 min. The reaction can be terminated when the desired color develops.
  3. Add 100 µl of stop solution (0.3 M H2SO4 or 0.25 M HCl) to each well.
  4. Read the absorbance of each well at 450 nm within 15 minutes.

Notes:
  • 0.1% NaF can be used as the stop solution instead of 0.3 M H2SO4 or 0.25 M HCl; the absorbance of each well should be read at 650 nm if using 0.1% NaF.
  • Avoid contamination with oxidizing reagents.
  • This product should be used in the dark. Do not use in metal containers.
  • High HRP concentrations may cause the TMB to turn a green or red color and may cause precipitation to occur. If this occurs, it is recommended to reduce the HRP concentration in the sample or reduce the incubation time.
Research Articles on TMB Substrate


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