Tyrosine-Protein Kinase ITK/TSK (ITK) Antibody

Tyrosine-Protein Kinase ITK/TSK (ITK) Antibody is a Rabbit Polyclonal antibody for the detection of ITK.

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.

Target	Tyrosine-Protein Kinase ITK/TSK (ITK)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/200 - 1/2000, IHC: 1/20 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	IL2-inducible T-cell kinase
Isotype	IgG
Form	Liquid
Purity	≥ 95% (SDS-PAGE)
Purification	Purified by immunogen affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Validity	12 months.
UniProt Primary AC	Q08881 (UniProt, ExPASy)
UniProt Secondary AC	B2R752, Q32ML7
UniProt Entry Name	ITK_HUMAN
Gene Symbol	ITK
GeneID	3702
OMIM	186973
HGNC	6171
KEGG	hsa:3702
Ensembl	ENSG00000113263
String	9606.ENSP00000398655
Molecular Weight	Observed MW: 68-72 kDa
Buffer	PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Concentration	2 mg/ml
Availability	Shipped within 5-12 working days.
Note	This product is for research use only.

Research Articles on Tyrosine-Protein Kinase ITK/TSK (ITK)

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