Ceramide Glucosyltransferase (UGCG) Protein
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Catalogue No: abx655856
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US$783.00
(Size: 50 µg)
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Datasheet SDS
Ceramide Glucosyltransferase (UGCG) Protein is an active protein expressed in E. coli. Ceramide Glucosyltransferase (UGCG) is an enzyme that catalyzes the first glycosylation step in glycosphingolipid biosynthesis. It belongs to the glycosyltransferase 2 family and is widely expressed with upregulated transcription during keratinocyte differentiation. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.
Research Articles on Ceramide Glucosyltransferase (UGCG)
| Target | Ceramide Glucosyltransferase (UGCG) |
| Research Area | Enzymes and Kinases, Metabolic Pathways, Endocrinology |
| Origin | Human, Mouse, Rat |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Exhibits binding activity. Enzymatic activity has not been tested. |
| Biological Activity | Binding activity of recombinant Ceramide Glucosyltransferase (UGCG) and recombinant Human Large Multifunctional Peptidase 2 (LMP2) was determined using an ELISA assay. UGCG was diluted serially in PBS, pH 7.4, with 0.01% BSA. 100 µl duplicates were then transferred to LMP2-coated microtiter wells and incubated for 2 hours at 37 °C. Wells were washed with PBST and incubated for 1 hour with a UGCG antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and fluorescence detected at 450 nm. The effect of binding activity of UGCG and LMP2 was determined to be in a dose dependent manner. |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| Gene Symbol | UGCG |
| Molecular Weight | Calculated MW: 16.1 kDa Observed MW (SDS-PAGE): 16 kDa |
| Sequence Fragment | Lys39-Leu171 |
| Sequence | KK ATDKQPYSKL PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK LLGKYPNVDA RLFIGGKKVG INPKINNLMP GYEVAKYDLI WICDSGIRVI PDTLTDMVNQ MTEKVGLVHG L |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl and 5% Trehalose. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Specificity | Cross-reactivity observed between Human, Mouse and Rat UGCG: 98%. |
| Concentration | Prior to lyophilization: 500 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |