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Urease Enzyme
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Catalogue No: abx073924
Price:
US$261.00
(Size: 1 mg)
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Datasheet SDS
Urease is a recombinant mutant enzyme expressed in E. coli. It is genetically designed to have a high Km to urea, which is suitable for kinetic urea assays with a wide measureable range. This enzyme is composed of three subunits: 60.3 kDa "a" subunit, 11.7 kDa "b" subunit and 11.1 kDa "g" subunit. The structure of is very similar to native microbial ureases.
Research Articles on Urease
| Target | Urease |
| Research Area | Enzymes and Kinases |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | E. coli |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | > 120 U/mg 1 Unit is defined as the amount of enzyme that oxidizes 1 µmol of NADH per minute at 25°C, pH 7.6. |
| Purity | > 90% (SDS-PAGE) |
| Purification | Sterile filtered. |
| Reconstitution | Reconstitute in sterile 18 MΩ · cm water. |
| Storage | Store lyophilized or reconstituted below -18°C for long-term storage. Store reconstituted at 4°C for up to 7 days. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| Stability | Stable at room temperature for up to 3 weeks. |
| Buffer | Each mg of protein contains 370 μg potassium phosphate and 30 μg EDTA Na2. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |