Vascular Endothelial Growth Factor A (VEGFA) Protein

Vascular Endothelial Growth Factor A (VEGFA) Protein (Active) is an active protein from Rat.

Target	Vascular Endothelial Growth Factor A (VEGFA)
Origin	Rat
Tested Applications	WB, SDS-PAGE
Host	293F cell
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 98%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P16612 (UniProt, ExPASy)
UniProt Secondary AC	Q541S6, Q91ZE1, Q9JKX7, Q9QXG6, Q9QXG7
Molecular Weight	Calculated MW: 20.8 kDa Observed MW: 22 kDa
Sequence Fragment	Ala27-Arg190
Tag	N-terminal His tag
Buffer	Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Activity	Active
Biological Activity	VEGFA is known to induce vascular permeability and growth, promote monocyte/macrophage migration, and inhibit cell apoptosis. To test the effect of VEGFA on cell proliferation of the ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFA. After incubation for 72 h, cells were observed using an inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, which was incubated for 1-4 hours at 37°C. The absorbance was then measured at 450 nm using a microplate reader. Cell proliferation of ECV304 cells after incubation with VEGFA for 72 h observed by inverted microscope is shown in Figure 4 and Figure 5. The dose-effect curve of VEGFA is shown in Figure 6, showing that VEGFA significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 5.58 to 9.98 ng/ml.
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Vascular Endothelial Growth Factor A (VEGFA)

Rat Vascular Endothelial Growth Factor A (VEGFA) Protein (Active)

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