Vimentin (VIM) Antibody

REQUEST MORE INFO
Catalogue No: abx016062
Price: US$420.50
(Size: 100 µl)

Click on the image to see the image legend

Available Options

* Size:

Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
Vimentin, also know as VIM.It is the major subunit protein of the intermediate filaments of mesenchymal cells.It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane.Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets.Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event.This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative.Melanomas, lymphomas and vascular tumors may all stain for Vimentin.Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors.They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Target Vimentin (VIM)
Clonality Monoclonal
Reactivity Human, Monkey
Tested Applications ELISA, WB, IHC
Host Mouse
Recommended dilutions ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Purified recombinant fragment of Vimentin (aa2-466) expressed in E. coli.
Isotype IgG1
Form Liquid
Purification Unpurified ascites.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
GeneID 7431
Molecular Weight 54 kDa
Buffer Ascitic fluid containing 0.03% sodium azide.
Concentration Not determined.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Research Articles on Vimentin (VIM)


Write a review