Spin Column Viral DNA/RNA Extraction Kit

Abbexa’s Spin Column Viral DNA/RNA Extraction Kit provides a fast and easy column-based method to isolate viral DNA and RNA from a variety of samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, whole blood, nasopharyngeal/oropharyngeal/tracheal aspirates, bronchoalveolar lavage fluid, sputum, nasopharyngeal/oropharyngeal swab samples and other cell-free biological fluids.

Viruses in samples are lysed using a lysis buffer and the released DNA / RNA is bound to silica-membrane. After washing, high purity DNA / RNA is eluted from the column, and is suitable for use in PCR, RT-PCR, qRT-PCR, qPCR and other downstream applications.

Kit Components:

Component	50 rxns	200 rxns
Binding Buffer	15 ml	60 ml
Wash Buffer	12 ml	2 × 24 ml
Proteinase K (20 mg/ml)	1 ml	4 × 1 ml
RNase-free Water	10 ml	20 ml
RNase-free Centrifuge Tubes (1.5 ml)	50 tubes	200 tubes
RNA Spin Columns with Collection Tubes	50 columns	200 columns

Target	Spin Column Viral DNA/RNA Extraction Kit
Storage	Store at room temperature.
Shelf Life	12 months.
Sample Collection/Preparation	Store samples at -70°C for long term storage, or at 4°C for up to 72 hours. Avoid repeated freeze/thaw cycles. It is recommended to collect swab samples using synthetic swab tips with aluminium or plastic shafts to avoid contamination.
Availability	Shipped within 10-20 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use	Assay Procedure: Add anhydrous ethanol to Wash Buffer at the following concentrations: 50 rxns: 48 ml 200 rxns: 96 ml (to both tubes) Sample Processing: Liquid Samples: Add 20 µl Proteinase K to a sterile 1.5 ml microcentrifuge tube. Add 200 µl Binding Buffer and vortex for 15 seconds. If using multiple samples, use the above ratio and separate into 220 µl aliquots. Add 200 µl sample to the tube and vortex for 15 seconds. If the sample volume is less than 200 µl, add PBS or 0.9% NaCl to bring the total volume to 200 µl. Incubate at 15°C for 15 minutes. Add 250 µl anhydrous ethanol (the sample may coagulate), vortex for 15 seconds and incubate at room temperature for 5 minutes. Solid Samples (swabs): Place the sample swab into a sterile 1.5 ml centrifuge tube containing the storage buffer. Add 300 µl Proteinase K and vortex. Incubate at 56°C for 20 minutes, vortexing 3-5 times during incubation. Remove the swab from the tube, centrifuge briefly and add 300 µl anhydrous ethanol (the sample may coagulate), vortex for 15 seconds and incubate at room temperature for 5 minutes. DNA/RNA Extraction: Transfer the sample into the provided spin column, and centrifuge at 12000 g for 1 minute. Discard the flow through. Load samples twice if the total volume is > 650 µl. Add 500 µl Wash Buffer and centrifuge at 12000 g for 1 minute. Discard the flow through. Repeat this step once. Centrifuge at 12000 g for 1 minute to remove the residual ethanol completely. Place the spin column in a new RNase-free tube and add 20-50 µl RNase-free water to the center of the column. Incubate at room temperature for 1 minute. Centrifuge at 12000 g for 1 minute to elute the DNA/RNA. Store eluted DNA at -20°C, and the RNA at -70°C. Notes: Carry out all centrifugation steps at room temperature. Ensure any anhydrous ethanol has been added to the Wash Buffer before use.

Research Articles on Spin Column Viral DNA/RNA Extraction Kit

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