Human Vitamin D Receptor (VDR) Protein (Active)

Human Vitamin D Receptor (VDR) Protein (Active) is an active protein from Human.

Vitamin D Receptor (VDR) is a nuclear hormone receptor for Vitamin D3. It belongs to the trans-acting transcriptional regulatory factor family and shows sequence similarity to the steroid and thyroid hormone receptors. It mediates the action of Vitamin D3 by controlling the expression of hormone-sensitive genes. . This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.

Target	Vitamin D Receptor (VDR)
Research Area	Metabolism, Endocrinology
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Activity	Active
Biological Activity	Measured using a binding ELISA assay between recombinant Human VDR and recombinant Human RXRa. Briefly, VDR were diluted serially in PBS, with 0.01% BSA, pH 7.4. 100 µl duplicates were transferred to RXRA-coated microtiter wells and incubated for 1h at 37 °C. Wells were aspirated and incubated for 1h with a VDR antibody and washed 3 times. After incubating with a HRP-conjugated secondary antibody, the wells were washed 5 times and substrate solution added. The wells were incubated for 15-25 minutes at 37 °C. 50 µL stop solution was added to the wells and read at 450 nm immediately. The binding activity was determined to be in a dose dependent manner and the EC50 for this effect was 0.469 µg/ml.
Purity	> 90%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Shelf Life	12 months.
UniProt Primary AC	P11473 (UniProt, ExPASy)
UniProt Secondary AC	B2R5Q1, G3V1V9, Q5PSV3
UniProt Entry Name	VDR_HUMAN
KEGG	hsa:7421
String	9606.ENSP00000447173
Molecular Weight	Calculated MW: 21.6 kDa Observed MW: 20 kDa
Sequence Fragment	Met272-Ser427
Tag	N-terminal His tag
Buffer	Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Endotoxin Level	< 1.0 EU/µg protein (LAL method)
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

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Human Vitamin D Receptor (VDR) Protein (Active)

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