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Wee1-Like Protein Kinase (WEE1) Cell ELISA Kit
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Catalogue No: abx595628
Price:
US$572.75
(Size: 96 tests)
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WEE1 Cell ELISA Kit is a cell-based ELISA Kit. Cells to be assayed should be seeded onto a clear flat bottom 96 well plate, using poly-L-lysine for non-adherent cells. Cells should be grown to 75-90% confluence and treated prior to carrying out the ELISA.
Research Articles on Wee1-Like Protein Kinase (WEE1)
| Introduction | WEE1 G2 Checkpoint Kinase (WEE1) is a protein that acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. It specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. |
| Target | Wee1-Like Protein Kinase (WEE1) |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | 6 months. |
| UniProt Primary AC | P30291 (UniProt, ExPASy) |
| UniProt Secondary AC | B3KVE1, D3DQV0 |
| UniProt Entry Name | WEE1_HUMAN |
| Gene Symbol | WEE1 |
| GeneID | 7465 |
| OMIM | 193525 |
| HGNC | 12761 |
| KEGG | hsa:7465 |
| Ensembl | ENSG00000166483 |
| String | 9606.ENSP00000402084 |
| Detection Method | Colorimetric |
| Plate Coating | Uncoated |
| Substrate | TMB |
| Detection Antibody Conjugation | HRP |
| Availability | Shipped within 1-2 weeks. |
| Note | This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
