Western Blot Blocking Solution and Signal Enhancer

Catalogue No: abx299719
£240.00 £150.00
(Size: 2 × 500 ml)
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Western Blot Blocking Solution and Signal Enhancer is a 3-in-1 solution, providing blocking, primary and secondary antibody hybridization, and enhancing the signal developed by horseradish peroxidase (HRP) or alkaline phosphatse (AP) substrates. One 500 ml bottle is suitable for use with over 25 mini-gel size membranes.

Material required but not provided:
  • Primary antibody
  • HRP-conjugated secondary antibody
  • Wash buffer: Phosphate buffered saline with Tween-20 (PBST) or Tris-buffered saline with Tween-20 (TBST)
  • Enhanced chemiluminescence (ECL) or colorimetric reagents


Target Western Blot Blocking Solution and Signal Enhancer
Tested Applications WB
Storage Store at 4 °C for up to 12 months.
Buffer Contains < 5% non-hazardous anionic surfactants.
Directions for use
  1. After Western blot transferring, immerse the PVDF or NC membrane in PBST buffer for 5 minutes.
  2. Dilute the primary antibody and secondary antibody with the appropriate amount of Western Blot Blocking Solution and Signal Enhancer (e.g. when the dilution factor for both primary and secondary antibodies is 1/10,000, add 2 µl of the primary antibody to 10 ml of Western Blot Blocking Solution and Signal Enhancer into a tube, followed by adding 2 µl of the secondary antibody to another 10 ml of the Western Blot Blocking Solution and Signal Enhancer into a second tube).
  3. Thoroughly mix the combined antibody-Western Blot Blocking Solution and Signal Enhancer solution inside each tube by inverting it back and forth.
  4. Pour the primary antibody-Western Blot Blocking Solution and Signal Enhancer solution into the prepared container first, followed by the addition of the secondary antibody-Western Blot Blocking Solution and Signal Enhancer solution into the same container.
  5. Incubate the membrane immediately in the antibody-Western Blot Blocking Solution and Signal Enhancer solution at room temperature for 1-2 hours with gentle agitation. Please notethat after mixing the primary and secondary antibodies, the membrane needs to be immediately immersed in the mixture within 10 minutes to obtain optimal performance.
  6. Wash the membrane with PBST/TBST three times with gentle shaking.
  7. Drain excessive wash buffer and perform image development methods with ECL or colorimetric system immediately.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
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