Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity)

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Catalogue No: abx460005
Price: US$348.00
(Size: 100 ml)
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Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity)

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Datasheet
Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity), a luminol-based chemiluminescent substrate is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP)-conjugated secondary antibodies. It is designed for the detection of the target proteins in amounts that are too small to be seen with typical ECL substrates.

Target Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity)
Tested Applications WB
Storage Store at room temperature for up to 24 months. Avoid exposure to light.
Buffer Not applicable.
Availability Shipped within 10-15 working days.
Note This product is for research use only.
Directions for use
  1. Keep the membrane moist in the wash buffer while preparing the substrate mixture. Please ensure the membrane does not dry out during the subsequent steps.
  2. Mix Luminol solution and Peroxide Solution in a 1:1 ratio, and thoroughly mix the chemiluminescent substrate solution well.
  3. Prepare 0.1 ml of solution per cm2 of membrane. For a mini-sized membrane (7 × 8.5 cm), 5 ml of solution is sufficient. For a midi-sized membrane (8.5 × 13.5 cm), 10 ml of solution is sufficient.
  4. Place the membrane with the protein side up on a clear and level surface or in a clean container.
  5. Remove the membrane from the chemiluminescent substrate solution and drain off excessive solution.
  6. Place the membrane in a plastic sheet protector or in plastic wrap to prevent the membrane from drying.
  7. Image the membrane with a digital imager or by exposure to X-ray film.
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