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Target | Tyrosine-Protein Kinase ZAP-70 (ZAP-70) |
Clonality | Monoclonal |
Clone | T052 |
Reactivity | Human |
Tested Applications | WB, IF/ICC, FCM |
Host | Mouse |
Recommended dilutions | WB: 0.5-2 µg/ml, FCM:2-5 µg/ml. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of Human ZAP-70 with histidine tag. |
Isotype | IgG1 |
Purity | > 95% (SDS-PAGE) |
Purification | Purified by Protein A affinity chromatography. |
Storage | Store at 2-8°C. Do not freeze. |
UniProt Primary AC | P43403 (UniProt, ExPASy) |
Gene Symbol | ZAP70 |
GeneID | 7535 |
KEGG | hsa:7535 |
String | 9606.ENSP00000264972 |
Buffer | PBS solution with 15 mM sodium azide. |
Specificity | The antibody reacts with ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products. |
Concentration | 1 mg/ml |
Availability | Shipped within 5-12 working days. |
Note | This product is for research use only. |