ZAP70 (pY319) Antibody

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Catalogue No: abx000514
Price: US$232.00
(Size: 20 µl)

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Immunoprecipitation analysis of 200 µg extracts of Jurkat cells, using 3 µg Phospho-ZAP70-Y319 antibody. Western blot was performed from the immunoprecipitate using Phospho-ZAP70-Y319 antibody at a dilution of 1/1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight.
ZAP70 (pY319) Antibody

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Datasheet SDS
ZAP70 (pY319) Antibody is a Rabbit Polyclonal antibody against ZAP70 (pY319). This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Target ZAP70 (pY319)
Clonality Polyclonal
Reactivity Human
Tested Applications ELISA, WB, IP
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/2000, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic phosphorylated peptide around Y319 of human ZAP70.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Shelf Life 12 months.
UniProt Primary AC P43403 (UniProt, ExPASy)
UniProt Secondary AC A6NFP4, Q6PIA4, Q8IXD6, Q9UBS6
UniProt Entry Name ZAP70_HUMAN
Gene Symbol ZAP70
GeneID 7535
NCBI Accession NP_001070.2
KEGG hsa:7535
String 9606.ENSP00000264972
Molecular Weight Calculated MW: 70 kDa
Observed MW: 70 kDa
Buffer PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on ZAP70 (pY319)


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