Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19) Spike S1 Protein Receptor-Binding Domain (RBD) is a recombinant protein expressed in Insect cells.

The SARS-CoV-2 Spike Protein (S protein) is a viral protein that allows the entry of SARS-CoV-2 into human cells. The protein forms trimers on the viral capsid and binds to human Angiotensin Converting Enzyme 2 (ACE2) located on the cell surface. The protein has a cleavage site between the S1 and S2 subunits that is targeted by the human enzyme Furin, and it may also cause the development of a syncytium (cell fusion). Antibodies to S protein can prevent viral entry as well as target the virus for further immune action.

The Gamma (P.1) variant was first identified in Brazil. It contains the K417T, E484K and N501Y mutations in the spike protein's receptor-binding domain.

Target	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) S1 Protein RBD (Gamma B.1.1.28.1 Variant)
Target Modification	Lys417Thr, Glu484Lys, Asn501Tyr
Modification	Mutation
Origin	Virus
Expression	Recombinant
Tested Applications	ELISA, WB
Host	Insect
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Form	Liquid
Purity	≥ 90% (SDS-PAGE)
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 26 kDa Observed MW (12% reducing SDS-PAGE): ~28 kDa
Sequence Fragment	319-541 AA.
Tag	C-terminal His tag
Buffer	PBS, pH 7.4. Does not contain preservatives.
Concentration	2.9 mg/ml
Availability	Shipped within 10-20 working days.
Note	This product is for research use only. This product is shipped with dry ice.
Directions for use	Centrifuge before opening to ensure complete recovery of vial contents.

Research Articles on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) S1 Protein RBD (Gamma B.1.1.28.1 Variant)

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